Polymerase Chain Reaction - Walter Scott, Jr. College of ...

Polymerase Chain Reaction - Walter Scott, Jr. College of ...

Polymerase Chain Reaction PCR PCR is one of those inventions like the internet, once you have used it, you cannot quite

understand how people managed before it existed Nobel Prize 1993 Invented by Dr. Kary Mullis,

Research Scientist, Perkin Elmer Cetus Corporation in April 1983. What is PCR?

A method of amplifying specific DNA sequences. A test tube system for DNA replication that allows a target DNA sequence to be selectively amplified several million fold in just a few hours.

How Does PCR Work? http://www.abpischools.org.uk/resources/p oster-series/pcr/pcranim.asp HOW DOES PCR WORK ?

PCR Reaction:

Template DNA Primers dNTPs Buffer

Taq DNA polymerase PCR Applications Endless. Problems with PCR

To be discussed. Example: Bias GC rich sequences tend to be preferentially amplified over AT rich sequences Other sources of bias?

Discussion Paper #1: Saiki et al. (Mullis) Science Paper #2: Bruce et al. Presented by Mary Beth Paper #3: Abdulmajwood et al.

Paper #4: Wang and Wang

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